Defense Date

2005

Document Type

Thesis

Degree Name

Master of Science

Department

Physiology

First Advisor

Dr. Roland N. Pittman

Abstract

Hemoglobin-based oxygen carriers for use as transfusion fluids have emerged as a leading technology directed at stemming shortages of a safe blood supply and providing a readily available resuscitation fluid in various trauma situations. The purpose of this investigation was to determine the effects of isovolemic hemodilution with Hespan and a hemoglobin-based oxygen carrier (HBOC) on erythrocyte-associated transients (EATs) in capillary PO2. The particulate nature of blood flow in capillaries, when observed from a stationary observation point, results in fluctuations of PO2 as alternating red blood cells and plasma gaps move through the detection region. Therefore, through experimental methods which provided the necessary temporal and spatial resolution required to make such measurements, EATs can be observed and corresponding PO2 fluctuations can be determined. The spinotrapezius muscle in sixteen Sprague-Dawley rats was exteriorized for intravital microscopy measurements in capillaries. Capillary PO2 was measured using Pd-porphyrin phosphorescence quenching microcopy. The hemodiluents used in isovolemic hemodilution included Hespan, a non-oxygen carrying plasma expander, and Oxyglobin®, a HBOC. Two isovolemic hemodilution steps were performed, reducing the systemic hematocrit to an average of 27.5% after the first step and 13.5% after the second step. Results showed that erythrocyte-associated transients in PO2 can be observed in the rat spinotrapezius with significant differences occurring between red blood cell and plasma gap PO2 under control conditions, isovolemic hemodilution with Oxyglobin after step one, and isovolemic hemodilution with Hespan after step two. This study concludes that EATs are observable and PO2 transients relating to EATs can be measured in the rat spinotrapezius muscle. Furthermore, it can be concluded that the HBOC Oxyglobin caused a decrease in erythrocyte-associated capillary PO2 transients, as well as a general decrease in capillary PO2. In addition, this study concludes that erythrocyte-associated capillary PO2 transients can best be observed under control conditions and after step two of isovolemic hemodilution with Hespan.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

June 2008

Included in

Physiology Commons

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