Defense Date

2010

Document Type

Thesis

Degree Name

Master of Science

Department

Physiology

First Advisor

Zendra Zehner

Abstract

Previously, the CCR5 receptor was found to be a good target for treating prostate cancer (PCa). Dr. Yan Zhang’s laboratory designed several CCR5 antagonists, which were screened for their inhibitory effect on the growth and invasion of the M12, DU145 and PC-3 PCa cell lines. Primary in vitro screening showed one compound (Drug 17) significantly inhibited the proliferation of PCa cells at 1μM concentration, with a half-maximal inhibitory concentration of 237.68 nM. Further in vitro assays including a proliferation, cytotoxicity and invasion assay confirmed the inhibitory effect of drug 17. The physiological effect of drug 17 was tested by the Ware laboratory in vivo by subcutaneous injection of M12 cells into male, athymic nude mice. Tumor growth was slowed in mice receiving injections of drug 17 compared to sham injected controls. Thus, in vitro and in vivo assays suggest drug 17 might be an effective therapy to block PCa progression.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

August 2010

Included in

Physiology Commons

Share

COinS