Defense Date

2014

Document Type

Thesis

Degree Name

Master of Science

Department

Microbiology & Immunology

First Advisor

S. Esra Sahingur

Abstract

Periodontitis is a biofilm-initiated inflammatory disease, resulting in soft tissue damage and alveolar bone loss. A nucleic acid sensor, toll-like receptor 9 (TLR9), has been recently implicated in periodontal inflammation. This study utilized an in vivo periodontitis model using TLR9 knockout (TLR9-/-) mice to assess the role of TLR9 in periodontitis. Significant bone loss was observed in wild type, but not in TLR9-/- mice. Further experiments using ex vivo assays revealed significantly higher IL-6 production in splenocytes of wild type mice compared to knockout cells in response to Porphyromonas gingivalis (a keystone pathogen for periodontitis) challenge. In conclusion, TLR9 contributes to periodontal inflammation through promoting a heightened inflammatory response. Therapeutics targeted to TLR9 may be beneficial to control periodontal disease.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

8-18-2014

Available for download on Thursday, August 15, 2024

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