DOI
https://doi.org/10.25772/D644-A616
Defense Date
2014
Document Type
Thesis
Degree Name
Master of Science
Department
Engineering
First Advisor
Vamsi Yadavalli
Abstract
Challenges in the development of successful cell therapies involve engineering and control of cues to regulate the balance between differentiation and self-renewal. However, the complexity of architecture and function make this an intriguing problem in the context of forming functional connections. Here we present the design and fabrication of microstructured scaffolds that present a biomimetic framework along which neural cell lines can organize into oriented constructs. Specifically, we show microfabricated non-linear architectures that promote cellular fate related to propagation of human neuroblastoma cells and directed differentiation towards neurons. By mimicking biological networks that allow for spreading of the cells instead of confining them in a groove or a well, a nonlinear configuration can promote a relaxed, self-supportive cell niche. The tailoring of non-homogeneous adhesion sites via the geometry and the compliance and roughness of the substrate allows a versatile microenvironment that promotes propagation and neuronal differentiation.
Rights
© The Author
Is Part Of
VCU University Archives
Is Part Of
VCU Theses and Dissertations
Date of Submission
8-19-2014