Defense Date

2015

Document Type

Thesis

Degree Name

Master of Science

Department

Microbiology & Immunology

First Advisor

Richard Marconi

Second Advisor

Jason Carlyon

Third Advisor

Daniel Conrad

Fourth Advisor

Darrell Peterson

Abstract

Lyme disease (LD) is the leading arthropod-borne disease in North America with 300-600,000 cases each year. There are currently no approved human LD vaccines. Outer surface protein C (OspC) has emerged as a leading vaccine candidate and an attractive diagnostic marker due to its antigenicity and expression early in infection. Several chimeric, epitope based OspC derived proteins were generated. The constructs were found to be highly immunogenic in mice and vaccination induced complement-dependent bactericidal antibodies. These results suggest that a broadly protective polyvalent OspC epitope based vaccine can be produced. Currently, LD diagnostic approaches are unreliable and unable to differentiate between early and late stage disease. An Ab response to OspE family proteins occurs later in infection. The two-Ag diagnostic assay using chimeric OspC proteins and a site-directed mutant of an OspE paralog, accurately differentiated between early and late infection in experimentally infected canines and humans.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

5-12-2015

Available for download on Friday, May 09, 2025

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