Determining the effect of knocking out microRNA-21 on subsarcolemmal and interfibrillar mitochondria
DOI
https://doi.org/10.25772/25NX-YS97
Defense Date
2016
Document Type
Thesis
Degree Name
Master of Science
Department
Physiology and Biophysics
First Advisor
Edward Lesnefsky
Second Advisor
Robert Diegelmann
Third Advisor
Roland Pittman
Abstract
Type 2 diabetes mellitus is a growing problem across the world and has significant pathological changes associated with it, including diabetic cardiomyopathy, wherein cardiac function is reduced. MicroRNA-21 has been shown to play a role in both the heart and diabetes so it was thought that knocking out miR-21 could have a protective effect on oxidative phosphorylation function in diabetic mice. Subsarcolemmal and interfibrillar mitochondria were isolated from adult male WT, miR-21 KO, db/db, and double knockout mice (db/db and miR-21 KO cross) and evaluated for function. Knocking out miR-21 in diabetic mice showed a restorative effect in Complex I and Complex II function even though it increased ROS production in Complex I and did not show a significant change in MPTP opening. Knocking out miR-21 could potentially restore oxidative phosphorylation function in diabetic patients but at the expense of producing more ROS.
Rights
© The Author
Is Part Of
VCU University Archives
Is Part Of
VCU Theses and Dissertations
Date of Submission
5-10-2016