Defense Date

2016

Document Type

Thesis

Degree Name

Master of Science

Department

Biology

First Advisor

Bruce D. Spiess

Second Advisor

Penny Reynolds

Third Advisor

Maryanne Collinson

Fourth Advisor

Gregory Walsh

Fifth Advisor

John Ryan

Abstract

EVALUATION OF REDOX POTENTIAL AS A NOVEL BIOMARKER OF OXIDATIVE STRESS, INFLAMMATORY RESPONSE, AND SHOCK USING NANOPOROUS GOLD ELECTRODES

Background: Redox potential is a chemical species’ affinity for electrons. Increased oxidant concentration is associated with disease1,2, yet there is not a way to measure systemic redox status.3 Redox potentiometry uses metal electrodes that do not work in blood because protein molecules adhere on the metal surface, blocking electron exchange.

Methods: Nanoporous gold electrodes have large surface areas that allowed electron exchange to continue in blood.4 Redox potential was measured in blood with ascorbic acid, in cardiac bypass patients and pigs undergoing hemorrhagic shock and resuscitation.

Results: Blood redox decreased with ascorbic acid addition, both in vitro and in vivo. It was more positive in patients undergoing cardiac surgery compared to healthy volunteers.

Conclusions: Preliminary studies were limited, but appear to show correlation to disease processes and medical therapies. More work needs to be done to further examine the relation of redox to disease and treatment.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

8-12-2016

Available for download on Tuesday, August 10, 2021

Included in

Biology Commons

Share

COinS