DOI
https://doi.org/10.25772/YR88-ZG32
Defense Date
2017
Document Type
Thesis
Degree Name
Master of Science
Department
Physiology and Biophysics
First Advisor
Stefano Toldo, Ph.D.
Abstract
There is a chronic lack of donor hearts to meet the need for heart transplant both in the US and worldwide. Further, the use of available hearts is limited by the short period between collection and implantation during which the heart can be safely preserved ex vivo. Using mid-thermic Langendorff machine perfusion, we have been able to preserve the metabolic function of a healthy heart for up to 8 hours, twice the limit for current static cold storage. We have also been able to preserve the metabolic function of a damaged DCD Heart collected 30 minutes after cardiac arrest for a period of 8 hours. We further investigated whether it was possible to improve the preservation of DCD heart using treatment with 10 μM Triiodothyronine to stimulate the tissue metabolism and we did find a reduction in damage markers in the treated DCD hearts as compared to the untreated group.
Rights
© William P. Adams
Is Part Of
VCU University Archives
Is Part Of
VCU Theses and Dissertations
Date of Submission
5-10-2017
Included in
Cardiology Commons, Circulatory and Respiratory Physiology Commons, Other Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons