Defense Date

2013

Document Type

Thesis

Degree Name

Master of Science

Department

Physics and Applied Physics

First Advisor

Joseph E Reiner

Abstract

Nanopores have been shown to be a useful analytical tool for single molecule detection. They have been used to study the composition of DNA and other molecules of interest. These pores are usually α-hemolysin which is a toxin from Staphylococcus aureus or more recently nanoscale synthetic solid state pores. Now we are beginning to look at other molecules or proteins by sending them into the nanopores and measuring a characteristic partial current blockade. In this thesis we look at polyethylene glycol (PEG) as it enters and blocks current through a single alpha hemolysin pore. We report the effects of ionic strength, PEG size, and applied voltage on the depth and duration of the current blockades. We also apply autocorrelation analysis on the arrival times of PEG molecules to the pore see if we can identify if the PEG is translocating through the pore or escaping from the same side it enters. This suggests a new approach to current blockade analysis.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

5-13-2013

Available for download on Sunday, May 13, 2018

Included in

Physics Commons

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