DOI
https://doi.org/10.25772/SC8X-WN96
Author ORCID Identifier
https://orcid.org/0000-0002-1154-6637
Defense Date
2018
Document Type
Thesis
Degree Name
Master of Science
Department
Chemistry
First Advisor
Dr. Indika U. Arachchige
Abstract
The detection and analysis of lipids in biological matrices for clinical applications poses many challenges, but rapid and reliable detection will prove invaluable for clinical diagnosis. Herein, we report the application of drop-casted Ag nanoplatelets as surface enhanced Raman scattering (SERS) substrates for qualitative detection of 20-hydroxyeicosatetraenoic acid (20-HETE), which is a potential biomarker for diagnosis of hypertensive disorders. Biomarker peaks of 20-HETE can be reliably detected and differentiated from those of the structurally similar lipids (arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid) commonly found in human blood, even 1 pM concentrations. Additionally, one study mixed 20-HETE with three structurally similar lipids at concentrations several orders of magnitude greater than the target lipid and 20-HETE could still be detected under these conditions. These experiments demonstrate the viability of SERS for the rapid and reliable detection of endogenous bioactive lipids, which has significant clinical impact in enabling point of care diagnostics.
Rights
© The Author
Is Part Of
VCU University Archives
Is Part Of
VCU Theses and Dissertations
Date of Submission
7-25-2018