Defense Date

2006

Document Type

Thesis

Degree Name

Master of Science

Department

Physiology

First Advisor

Dr. Helen L. Fillmore

Abstract

Malignant brain tumors are among the deadliest of human cancers. Despit recent advancements in conventional therapies, glioblastomas remain incurable, largel y due to their ability to invade surrounding tissue. Matrix metalloproteinases are thought to contribute to the invaseive phenotype of human gliomas. Absent in normal brain, matrix metalloproteinase-1 (MMP-1) has been shown to be present in gliomas, and in particular in glioblastoma multiforme (GBM). To begin to examine the role of MMP-1 in these tumore, two human glioma cell lines were stably transfected with MMP-1 cDNA. Confirmation of MMP-1 over-expression in these cells was achieved through real-time PCR and Western blot analysis. The functional consequences of MMP-1 over-expression were analyzed using a collagen type-I invasion assay along with clonogenic and ATP viability assays. Data presented demonstrate that MMP-1 over-expressing cells were more invasive in both cell types and interestingly more clonogenic in on of the glioma cell lines, supporting a possible role for MMP-1 in glioma growth and invasion.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

June 2008

Included in

Physiology Commons

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