Document Type
Article
Original Publication Date
2015
Journal/Book/Conference Title
Genes & Development
Volume
29
Issue
9
DOI of Original Publication
10.1101/gad.257568.114
Date of Submission
December 2015
Abstract
The DREAM complex represses cell cycle genes during quiescence through scaffolding MuvB proteins with E2F4/5 and the Rb tumor suppressor paralog p107 or p130. Upon cell cycle entry, MuvB dissociates from p107/p130 and recruits B-Myb and FoxM1 for up-regulating mitotic gene expression. To understand the biochemical mechanisms underpinning DREAM function and regulation, we investigated the structural basis for DREAM assembly. We identified a sequence in the MuvB component LIN52 that binds directly to the pocket domains of p107 and p130 when phosphorylated on the DYRK1A kinase site S28. A crystal structure of the LIN52–p107 complex reveals that LIN52 uses a suboptimal LxSxExL sequence together with the phosphate at nearby S28 to bind the LxCxE cleft of the pocket domain with high affinity. The structure explains the specificity for p107/p130 over Rb in the DREAM complex and how the complex is disrupted by viral oncoproteins. Based on insights from the structure, we addressed how DREAM is disassembled upon cell cycle entry. We found that p130 and B-Myb can both bind the core MuvB complex simultaneously but that cyclin-dependent kinase phosphorylation of p130 weakens its association. Together, our data inform a novel target interface for studying MuvB and p130 function and the design of inhibitors that prevent tumor escape in quiescence.
Rights
Copyright © 2015 Guiley et al.; Published by Cold Spring Harbor Laboratory Press This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
Is Part Of
VCU Internal Medicine Publications
Supplemental Figure 1. LIN52 and p130 expressed in Sf9 cells are phosphorylated. Supplemental Figure 2. Comparison of p107 and Rb structures. Supplemental Figure 3. Electron density surrounding the E721-29, LIN5212-34;phosS28, and LIN5212- 34;P29A;phosS28 peptides. Supplemental Figure 4. Location of missense mutations in p107 that occur in human cancer.
Comments
Originally published at http://dx.doi.org/10.1101/gad.257568.114