Live Cell Interferometry Quantifies Dynamics of Biomass Partitioning during Cytokinesis

Authors

Thomas A. Zangle, University of California
Michael A. Teitell, University of California
Jason Reed, Virginia Commonwealth UniversityFollow

Document Type

Article

Original Publication Date

2014

Journal/Book/Conference Title

PLOS ONE

Volume

9

Issue

12

DOI of Original Publication

10. 1371/journal.pone.0115726

Comments

Originally published at http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0115726.

Date of Submission

December 2016

Abstract

The equal partitioning of cell mass between daughters is the usual and expected outcome of cytokinesis for self-renewing cells. However, most studies of partitioning during cell division have focused on daughter cell shape symmetry or segregation of chromosomes. Here, we use live cell interferometry (LCI) to quantify the partitioning of daughter cell mass during and following cytokinesis. We use adherent and non-adherent mouse fibroblast and mouse and human lymphocyte cell lines as models and show that, on average, mass asymmetries present at the time of cleavage furrow formation persist through cytokinesis. The addition of multiple cytoskeleton-disrupting agents leads to increased asymmetry in mass partitioning which suggests the absence of active mass partitioning mechanisms after cleavage furrow positioning.

Rights

Copyright: 2014 Zangle et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Is Part Of

VCU Massey Cancer Center Publications