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The equal partitioning of cell mass between daughters is the usual and expected outcome of cytokinesis for self-renewing cells. However, most studies of partitioning during cell division have focused on daughter cell shape symmetry or segregation of chromosomes. Here, we use live cell interferometry (LCI) to quantify the partitioning of daughter cell mass during and following cytokinesis. We use adherent and non-adherent mouse fibroblast and mouse and human lymphocyte cell lines as models and show that, on average, mass asymmetries present at the time of cleavage furrow formation persist through cytokinesis. The addition of multiple cytoskeleton-disrupting agents leads to increased asymmetry in mass partitioning which suggests the absence of active mass partitioning mechanisms after cleavage furrow positioning.
Zangle, T. A., Teitell, M. A., & Reed, J. Live Cell Interferometry Quantifies Dynamics of Biomass Partitioning during Cytokinesis. PLoS ONE, 9, e0115726. Copyright © 2014 Zangle et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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VCU Physics Publications
LCI data from a single dividing L cell pair.
S1_Movie.mp4 (1601 kB)
Movie of cell division shown in Fig. 2A. Movie of symmetrically dividing mouse L cell. Red outline shows the identified outline of cell by the watershed transform.
S2_Fig.tif (1031 kB)
Averaged relative mass and mean phase shift data for control (untreated) mouse L cells.
S2_Movie.mp4 (1740 kB)
Movie of cell division shown in Figure 2C. Movie of asymmetrically dividing mouse L cell. Red outline shows the identified outline of cell by the watershed transform.
S3_Fig.tif (636 kB)
Effect of culture confluence on cell division asymmetry.
S3_Movie.mp4 (4033 kB)
Movie of cell division shown in Fig. 2E. Movie of dividing L cell showing change from asymmetric to symmetric mass partitioning.
S4_Fig.tif (660 kB)
Cell tracking results from drug treatment experiments.
S4_Movie.mp4 (7065 kB)
Movie of cell division shown in Fig. 2G. Movie of dividing L cell showing change from symmetric to asymmetric mass partitioning.
S5_Fig.tif (1419 kB)
Shape factor dynamics during cell division with cytoskeletal inhibitors.