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Abstract

ABSTRACT Virus induced gene silencing (VIGS) is an efficient technique to quickly evaluate gene functions. VIGS utilizes the mechanism(s) of RNA interference (RNAi), which the virus vector carrying the gene of interest will be silenced through posttranscriptional gene silencing. Agrobacterium is used to carry the constructed vectors to infect the plants . Here, I summarized several inoculation methods have been reported recently to infect the plants. We are working on evaluating the efficiency with these different methods in our study system. Our lab uses Nicotiana obtusifolia (desert tobacco) as a model to studygene regulating plant development. I report the result of leaf inoculation by using syringe. PDS gene was silenced show a phenotype with bleached leaves . We are working on evaluating the most effective inoculation methods for our system. VIGS, PDS, plasmid, agrobacterium, inoculation, vector, transmission

Publication Date

2014

Subject Major(s)

Genetics

Current Academic Year

Senior

Faculty Advisor/Mentor

Wenheng Zhang

Sponsorship

Virginia Commonwealth University. Undergraduate Research Opportunities Program

Is Part Of

VCU Undergraduate Research Posters

Rights

© The Author(s)

VIGS (Virus Induced Gene Silencing)

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