Document Type


Original Publication Date


Journal/Book/Conference Title

BioMed Research International



DOI of Original Publication



Originally published at

Date of Submission

December 2015


This study assessed contributions of micron-scale topography on clinically relevant titanium (Ti) to differentiation of osteoprogenitor cells and osteoblasts; the interaction of this effect with 1𝛼,25-dihydroxyvitamin D3 (1𝛼,25(OH)2D3); and if the effects are sex-dependent. Male and female rat bone marrow cells (BMCs) were cultured on acid-etched (A, π‘…π‘Ž = 0.87 πœ‡m), grit-blasted (GB, π‘…π‘Ž = 3.90 πœ‡m), or grit-blasted/acid-etched (SLA, π‘…π‘Ž = 3.22 πœ‡m) Ti. BMCs were sensitive to surface topography and underwent osteoblast differentiation. This was greatest on SLA; acid etching and grit blasting contributed additively. Primary osteoblasts were also sensitive to SLA, with less effect from individual structural components, demonstrated by enhanced local factor production. Sex-dependent responses of BMCs to topography varied with parameter whereas male and female osteoblasts responded similarly to surface treatment. 1𝛼,25(OH)2D3 enhanced cell responses on all surfaces similarly. Effects were sex-dependent and male cells grown on a complex microstructured surface were much more sensitive than female cells. These results indicate that effects of the complex SLA topography are greater than acid etching or grit blasting alone on multipotent BMCs and committed osteoblasts and that individual parameters are sex-specific. The effect of 1𝛼,25(OH)2D3 was sex dependent. The results also suggest that levels of 1𝛼,25(OH)2D3 in the patient may be important in osseointegration.


Copyright Β© 2015 Rene Olivares-Navarrete et al.This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Is Part Of

VCU Biomedical Engineering Publications