Defense Date


Document Type


Degree Name

Master of Science


Human Genetics

First Advisor

Shawn Holt


Induced Pluripotent Stem cells (iPScs) are artificially generated cells that demonstrate multilineage differentiation potential. These cells demonstrate similar morphology and high differentiation potential to Embryonic Stem Cells (ESCs). Generation of these cells from a terminally differentiated cell line requires activation of the core pluripotency genes Nanog, Oct4, and Sox2 as well as an oncogenic stimulus such as c-Myc. Here we examine the effect of the Human Pappiloma Virus derived proteins E6 and E7 on the ability of a terminally differentiated fibroblast cell line to a more primitive state and examine its multilineage differentiation capacity. In this paper, we attempt to differentiate BJ hTERT fibroblasts into adipogenic and osteogenic lineages with and without the core pluripotency factors Nanog, Oct4, Sox2 and also c-Myc using non-integrative adenoviral infections. We review the potential mechanisms through which changes in differentiation capacity changes occur through examination of the effects of E6 on the tumor suppressor protein p53. We determined that the proteins E6 and E7 when stably infected into BJ hTERT fibroblasts increase induced differentiation into adipogenic and osteogenic lineages. E6 and E7 can be considered components for generating cells with multipotent capacity with the addition of as little as one core pluripotency factor.


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Date of Submission

August 2011