Defense Date

1983

Document Type

Dissertation

Degree Name

Doctor of Philosophy

Department

Chemistry

First Advisor

Syang Yang Su

Abstract

Room Temperature Phosphorimetry (RTP) is a sensitive and selective technique which is well suited to the analysis of compounds of environmental and pharmaceutical interests. Absolute sensitivity is generally in the low to subnanograrn range. Selectivity of this technique is due to the fact that only several hundred compounds phosphoresce at room temperature. In these studies attempts are made to gain a greater understanding of the phenomenon of solid substrate room temperature phosphorimetry and to extend the application range of the technique.

This goal is approached through several directions, all leading to a greater understanding of solid substrate luminescence in general, and in specific, Room Temperature Phosphorimetry. High Performance Thin Layer Chromatography (HPTLC) plates are evaluated as solid substrates for RTP. This yields the immediate benefit of a new and useful solid substrate for the analysis of ionic compounds. In addition a mechanism for RTP enhancement of ionic compounds and Polycyclic Aromatic Hydrocarbons (PAH) compounds is discussed.

The extension of the application range of RTP is approached by introducing derivatization reagents. By using fluorescamine, the application range of RTP is extended to include all primary amines. An investigation is carried out to determine limits of detection of various amino acids in laboratory prepared solutions. In addition, the technique is extended to include phosphorescent and non-phosphorescent pharmaceutical compounds, concluding with a real sample determination of phenylpropanolamine in diet capsules.

The development of a solid substrate luminescence detector for liquid chromatography also extends the application range of RTP. By developing such instrumentation, selectivity of both Room Temperature Phosphorescence and Fluorescence (RTP and RTF) and Liquid Chromatography (LC) can be employed together to minimize the amount of time needed for analysis. In addition, concentrating power of liquid chromatography can be employed in the analysis of more dilute solutions of analyte, in situations where sample volume is not a concern.

Lastly, solid substrate luminescence of pharmaceutical materials in tablet form is discussed. This investigation shows the feasibility of using Solid Substrate Luminescence techniques to analyze active ingredients in pharmaceutical preparations. In this study, propranolol, para amino benzoic acid and acetylsalicylic acid are used as model compounds to determine the feasibility of nearly non-destructive analysis using Solid Substrate Luminescence.

Comments

Scanned, with permission from the author, from the original print version, which resides in University Archives.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

1-23-2018

Included in

Chemistry Commons

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