Master of Science
Biochemistry and Molecular Biology
The objectives of this thesis were to examine serum vitamin A levels in two defined populations. The method of analysis was a spectrophotofluorometric technique with a correction formula to account for interfering fluorescence from an identified carotenoid phytofluene (37).
The first population were infants at time of delivery. Infants were divided into two groups depending upon length of gestation. A premature group was identified as having a length of gestation of less than 36 weeks. Term infants were defined as 36 weeks or greater. The mean value of serum vitamin A of eight premature infants was 10.5 μg/100ml (S.E.M.+.78). The mean value of 54 term infants was 22.5 μg/100ml (S.E.M.+.96). Comparing these two groups results in a p value of <0.001. The increased incidence of necrotizing entercolitis in premature infants, and the fact that vitamin A is important for the maintenance of a functional mucous membrane (28,20)gives significance to these findings. It is suggested in this thesis that the prophylactic administration of vitamin A to premature infants may be beneficial. The comparison of serum vitamin A levels of eight mothers and their infants resulted in a mean of 40.7 and 19.3 μg/100ml serum respectively. Statistical analysis revealed a p value of<0.005.
The second population studied were patients undergoing periodontal surgery. The purpose was to monitor serum vitamin A levels before and after surgery. The results are inconclusive due to the small number of samples. In this thesis the results are discussed and recommendations are made for future projects in this area.
© The Author
Is Part Of
VCU University Archives
Is Part Of
VCU Theses and Dissertations
Date of Submission