Defense Date

1976

Document Type

Thesis

Degree Name

Master of Science

Department

Biochemistry and Molecular Biology

First Advisor

Richard Brandt

Abstract

The objectives of this thesis were to examine serum vitamin A levels in two defined populations. The method of analysis was a spectrophotofluorometric technique with a correction formula to account for interfering fluorescence from an identified carotenoid phytofluene (37).

The first population were infants at time of delivery. Infants were divided into two groups depending upon length of gestation. A premature group was identified as having a length of gestation of less than 36 weeks. Term infants were defined as 36 weeks or greater. The mean value of serum vitamin A of eight premature infants was 10.5 μg/100ml (S.E.M.+.78). The mean value of 54 term infants was 22.5 μg/100ml (S.E.M.+.96). Comparing these two groups results in a p value of <0.001. The increased incidence of necrotizing entercolitis in premature infants, and the fact that vitamin A is important for the maintenance of a functional mucous membrane (28,20)gives significance to these findings. It is suggested in this thesis that the prophylactic administration of vitamin A to premature infants may be beneficial. The comparison of serum vitamin A levels of eight mothers and their infants resulted in a mean of 40.7 and 19.3 μg/100ml serum respectively. Statistical analysis revealed a p value of<0.005.

The second population studied were patients undergoing periodontal surgery. The purpose was to monitor serum vitamin A levels before and after surgery. The results are inconclusive due to the small number of samples. In this thesis the results are discussed and recommendations are made for future projects in this area.

Comments

Scanned, with permission from the author, from the original print version, which resides in University Archives.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

4-5-2018

Included in

Biochemistry Commons

Share

COinS