Author ORCID Identifier

https://orcid.org/0000-0002-1154-6637

Defense Date

2018

Document Type

Thesis

Degree Name

Master of Science

Department

Chemistry

First Advisor

Dr. Indika U. Arachchige

Abstract

The detection and analysis of lipids in biological matrices for clinical applications poses many challenges, but rapid and reliable detection will prove invaluable for clinical diagnosis. Herein, we report the application of drop-casted Ag nanoplatelets as surface enhanced Raman scattering (SERS) substrates for qualitative detection of 20-hydroxyeicosatetraenoic acid (20-HETE), which is a potential biomarker for diagnosis of hypertensive disorders. Biomarker peaks of 20-HETE can be reliably detected and differentiated from those of the structurally similar lipids (arachidonic acid, eicosapentaenoic acid, and docosahexaenoic acid) commonly found in human blood, even 1 pM concentrations. Additionally, one study mixed 20-HETE with three structurally similar lipids at concentrations several orders of magnitude greater than the target lipid and 20-HETE could still be detected under these conditions. These experiments demonstrate the viability of SERS for the rapid and reliable detection of endogenous bioactive lipids, which has significant clinical impact in enabling point of care diagnostics.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

7-25-2018

Available for download on Wednesday, July 24, 2019

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