DOI
https://doi.org/10.25772/Y02R-0G39
Author ORCID Identifier
https://orcid.org/0000-0002-2090-4862
Defense Date
2018
Document Type
Dissertation
Degree Name
Doctor of Philosophy
Department
Integrative Life Sciences
First Advisor
Jason Reed
Second Advisor
Amir Toor
Third Advisor
John Ryan
Fourth Advisor
Masoud Manjili
Fifth Advisor
Joseph Reiner
Abstract
Cell mass is an important indicator of cell health and status. A diverse set of techniques have been developed to precisely measure the masses of single cells, with varying degrees of technical complexity and throughput. Here, the development of a non-invasive, label-free optical technique, termed Live-Cell Interferometry (LCI), is described. Several applications are presented, including an evaluation of LCI’s utility for assessing drug response heterogeneity in patient-derived melanoma lines and the measurement of CD3+ T cell kinetics during hematopoietic stem cell transplantation. The characterization of mast cells during degranulation, the measurement of viral reactivation kinetics in Kaposi’s Sarcoma, and drug response studies in patient-derived xenograft models of triple-negative breast cancer are also discussed. Taken together, data from these studies highlight LCI’s versatility as a tool for clinical, translational, and basic research applications.
Rights
© The Author
Is Part Of
VCU University Archives
Is Part Of
VCU Theses and Dissertations
Date of Submission
7-30-2018
Included in
Allergy and Immunology Commons, Biological and Chemical Physics Commons, Cancer Biology Commons, Cell Biology Commons, Hematology Commons, Integrative Biology Commons, Medical Biophysics Commons, Oncology Commons, Other Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons