Defense Date

2018

Document Type

Thesis

Degree Name

Master of Science

Department

Microbiology & Immunology

First Advisor

Richard T. Marconi

Abstract

Periodontal disease is a progressive inflammatory condition that is characterized by the reabsorption of alveolar bone, the destruction of connective tissue, and edentulism. It is caused by a dysbiosis in the oral microbiome as a result of a shift from a Gram-positive aerobic bacterial population, to one that becomes more Gram-negative and anaerobic. Treponema denticola is thought to drive this diseased state based on its role as a keystone periopathogen. A major component of T.denticola’s invasiveness is its motility, which allows the spirochete to penetrate and disseminate through tissues. This motility, which has been seen to be crucial to the invasiveness in other spirochetes through deletion studies, is often regulated by the second messenger, c-di-GMP. In this study, biochemical and biophysical assays were utilized to determine that the predicted diguanylate cyclase TDE0125 converts GTP to c-di-GMP. This elucidates further function of the c-di-GMP regulatory network in T. denticola.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

8-10-2018

Available for download on Wednesday, August 09, 2023

Included in

Bacteriology Commons

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