Defense Date

2018

Document Type

Thesis

Degree Name

Master of Science in Dentistry

Department

Dentistry

First Advisor

Dr. Zhao Lin

Second Advisor

Dr. Thomas Waldrop

Third Advisor

Dr. Harvey Schenkein

Abstract

Background: Exosomes have great potential in regenerative medicine through the

transfer of their bioactive cargos, such as RNA. tRF RNA and tiRNA are tRNAderived

non-coding RNA. Here, we sought to identify the tRF/tiRNA profile in human

mesenchymal stem cell (hMSC) exosomes. Methods: Bone marrow hMSCs were

cultured with/without osteogenic differentiation medium and exosomes were

harvested. RNA was extracted from: 1) control cells (Cell-NT); 2) control exosomes

(EXO-NT); 3) differentiated cells (Cell-OM); 4) exosomes produced by differentiated

cells (EXO-OM). RNA was sequenced to profile the small RNA with a focus on

tRF/tiRNA. Results: tRF/tiRNA was highly enriched in hMSC exosomes. Less

diversity was seen in the tRF/tiRNA profile in exosomes than that in parent cells.

Selective tRF/tiRNA were packed into MSC exosomes and their profile is dependent

on the cell maturation status. Conclusions: Our results suggest that tRF/tiRNA may

play a role in mediating the function of exosomes in tissue regeneration.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

11-12-2018

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