DOI
https://doi.org/10.25772/RDCK-D113
Defense Date
2019
Document Type
Thesis
Degree Name
Master of Science in Dentistry
Department
Dentistry
First Advisor
Sinem Esra Sahingur
Second Advisor
Thomas Waldrop
Third Advisor
Zhao Lin
Abstract
Purpose: To determine the effect of Quercetin, a plant based over the counter flavonoid, on periodontal inflammation in a murine model of periodontitis.
Methods: Periodontitis was induced in age and gender matched mice (C57BL/6) using ligatures on the left side of the maxilla and right side was left un-ligated as baseline control. The animals received daily Quercetin gavage or Phosphate Buffered Saline (PBS) starting 5 days prior to ligation which continued throughout the experimental protocol. Micro-computed tomography (micro CT) imaging was used to determine alveolar bone loss. Real Time quantitative (qRT) PCR was used to determine level of gingival tissue cytokine expression. Hematoxylin and eosin (H&E) staining was used to determine gingival tissue inflammatory cell infiltrate.
Results:There was significantly increased bone loss and cytokine (TNF and IL-6) expression in the ligated sites of both groups of mice compared to un-ligated control sites (p
Conclusion: Quercetin supplement diminishes gingival inflammation. Future studies are warranted to determine the beneficial effect of quercetin improving clinical outcomes in periodontitis patients.
Rights
© Sara Holden, DDS
Is Part Of
VCU University Archives
Is Part Of
VCU Theses and Dissertations
Date of Submission
4-29-2020