Defense Date

2021

Document Type

Dissertation

Degree Name

Doctor of Philosophy

Department

Chemistry

First Advisor

Heather Lucas

Abstract

Streptococcus sanguinis is an opportunistic bacterium living beneficially in the human mouth. When S. sanguinis gains access to the bloodstream, it can result in colonization on damaged tissue and generate heart valve vegetations that can develop into infective endocarditis. It has been suggested that Mn and Fe are cofactors in the pathogenic capacity of this bacterium, and that these redox active metals are imported by the metal binding lipoprotein S. sanguinis adhesin B (SsaB). The SsaB lipoprotein is an integral part of SsaACB, an ATP-binding cassette (ABC) transport system in S. sanguinis. When SsaB is deleted, the resulting strain displays decreased virulence. The principal goal of this research has been to demonstrate the importance and fundamental properties of SsaACB as a regulator of intracellular redox maintenance within S. sanguinis and to establish its role as a dual Fe/Mn importer. In doing so, we may inspire new approaches to control the virulence of S. sanguinis. As a facultative anaerobe, deriving the variations between aerobic and anaerobic intracellular redox homeostasis in S. sanguinis and the role SsaACB has allows for a potential targeted approach for future studies. Additionally, advanced studies analyzing SsaB structural changes and competitive metal binding are also underway to shed light on the biophysical properties of SsaB and the nuances of its role in metal transport and virulence in S. sanguinis. These studies provide insight into how SsaB governs S. sanguinis redox environment, bringing clarity to the molecular details of how SsaB interacts with key biometals and shedding light on its role in metal import.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

5-14-2021

Available for download on Wednesday, May 13, 2026

Share

COinS