Defense Date

2021

Document Type

Thesis

Degree Name

Master of Science

Department

Biology

First Advisor

Santiago Lima

Abstract

In my research, I developed tools and performed assays to study the in vitro transformation of immortalized lung epithelial cells by oncogenic KRASG12V. I delivered the KRAS by transfection and lentiviral transduction, and after confirming the cells expressed KRAS, I evaluated transformation using several experimental approaches including monitoring of EMT markers and various types of growth assays. I also conducted preliminary experiments that quantified sphingolipid alterations in the cells that had markers indicative of having gone through EMT. The goal of these experiments was to assess whether sphingolipids are altered in cells that are transformed in vitro by KRASG12V.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

5-13-2021

Available for download on Thursday, March 26, 2026

Included in

Biology Commons

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