DOI
https://doi.org/10.25772/2ZVM-RS47
Defense Date
2021
Document Type
Thesis
Degree Name
Master of Science
Department
Biology
First Advisor
Santiago Lima
Abstract
In my research, I developed tools and performed assays to study the in vitro transformation of immortalized lung epithelial cells by oncogenic KRASG12V. I delivered the KRAS by transfection and lentiviral transduction, and after confirming the cells expressed KRAS, I evaluated transformation using several experimental approaches including monitoring of EMT markers and various types of growth assays. I also conducted preliminary experiments that quantified sphingolipid alterations in the cells that had markers indicative of having gone through EMT. The goal of these experiments was to assess whether sphingolipids are altered in cells that are transformed in vitro by KRASG12V.
Rights
© The Author
Is Part Of
VCU University Archives
Is Part Of
VCU Theses and Dissertations
Date of Submission
5-13-2021