Defense Date

2022

Document Type

Thesis

Degree Name

Master of Science

Department

Microbiology & Immunology

First Advisor

Kimberly Jefferson

Abstract

Sneathia vaginalis is a gram-negative anaerobic bacterium that is associated with bacterial vaginosis, urethritis, preterm birth, pregnancy complications, and amnionitis. The only toxin that has been identified in this species is the cytopathogenic toxin A or CptA. CptA is a 2,310 amino acid pore-forming toxin that is hemolytic and cytotoxic; however, little is known about what it binds to on the host cell surface or which parts of the toxin are responsible for activity. In this study, we cloned truncated versions of the cptA gene into an expression vector and examined binding and pore-formation. The results suggest that there is a binding region between amino acids 1221 and 1320 and a pore-forming region between amino acids 24 and 147. Furthermore, CptA was found to bind to collagen.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

5-9-2022

Available for download on Saturday, May 08, 2027

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