MCV/Q, Medical College of Virginia Quarterly
Orginal Publication Date
1967
Journal Title
MCV/Q, Medical College of Virginia Quarterly
Volume
3
Issue
4
First Page
197
Last Page
205
Abstract
A number of years ago I decided to attempt to measure the intracellular pH of skeletal muscle by a more direct method than those in current use, that is, by an electrode. I excluded all forms of pH electrodes except the glass electrode and then set up a certain number of characteristics or requirements that this electrode should have. First, the electrode should be insulated in such a manner that only the pH-sensitive portion is in the cell cytoplasm. Second, the reference side of the electrode group should also be within the cell, because the cell has a transmembrane potential; and the only way to circumvent this transmembrane potential in actual electrical measurements is to have the reference electrode within the cell. Finally, the tip diameter of the combined pH and reference electrode should be less than 1 μ in order to reduce damage of the single cell by penetration or impalement. [Includes discussion.]
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