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The aim of this study is to observe cell proliferation, cell viability, apoptosis, and autophagy on acute myeloid leukemia (AML) cell lines, NB4 and U937, with the drug repurposing of mefloquine (MQ). Methods such as the 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay and trypan blue staining have shown a decrease in live cells with high concentrations of mefloquine. Using their average perspective IC50 values of MQ concentration, Western blotting was applied by means of apoptosis and autophagy markers to determine if the induction of apoptosis and inhibition of autophagy was present in MQ-treated AML cells. The experiment will be continued with more cell lines, drugs, and other means of protocol in order to contribute to cancer therapy.
Acute Myeloid Leukemia, AML, Mefloquine, Drug Repurposing, Autophagy Inhibition, Apoptosis Induction
Chemical Actions and Uses | Hematology | Hemic and Lymphatic Diseases | Medical Pathology | Oncology | Pediatrics
Current Academic Year
Dr. Seth Corey
© The Author(s)
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