Document Type
Article
Original Publication Date
2012
Journal/Book/Conference Title
The Journal of Chemical Physics
Volume
137
Issue
21
DOI of Original Publication
10.1063/1.4766363
Date of Submission
October 2015
Abstract
Over 15 years ago, the ability to electrically detect and characterize individual polynucleotides as they are driven through a single protein ion channel was suggested as a potential method for rapidly sequencing DNA, base-by-base, in a ticker tape-like fashion. More recently, a variation of this method was proposed in which a nanopore would instead detect single nucleotides cleaved sequentially by an exonuclease enzyme in close proximity to one pore entrance. We analyze the exonuclease/nanopore-based DNA sequencing engine using analytical theory and computer simulations that describe nucleotide transport. The available data and analytical results suggest that the proposed method will be limited to reading bases, imposed, in part, by the short lifetime each nucleotide spends in the vicinity of the detection element within the pore and the ability to accurately discriminate between the four mononucleotides.
Rights
Reiner, J. E., Balijepalli, A., & Robertson, J. W. F., et al. The effects of diffusion on an exonuclease/nanopore-based DNA sequencing engine. The Journal of Chemical Physics, 137, 214903 (2012). Copyright © 2012 American Institute of Physics.
Is Part Of
VCU Physics Publications
Comments
Originally published at https://dx.doi.org/10.1063/1.4766363