The Evaluation of Mefloquine Drug Repurposing on Acute Myeloid Leukemia

Authors

• Jessica L. Phan, Virginia Commonwealth UniversityFollow
• Bhavuk Garg, Massey Cancer Center of Virginia Commonwealth UniversityFollow
• Hrishikesh Mehta, Massey Cancer Center of Virginia Commonwealth UniversityFollow
• Seth Corey, Massey Cancer Center of Virginia Commonwealth UniversityFollow

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Abstract

The aim of this study is to observe cell proliferation, cell viability, apoptosis, and autophagy on acute myeloid leukemia (AML) cell lines, NB4 and U937, with the drug repurposing of mefloquine (MQ). Methods such as the 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay and trypan blue staining have shown a decrease in live cells with high concentrations of mefloquine. Using their average perspective IC50 values of MQ concentration, Western blotting was applied by means of apoptosis and autophagy markers to determine if the induction of apoptosis and inhibition of autophagy was present in MQ-treated AML cells. The experiment will be continued with more cell lines, drugs, and other means of protocol in order to contribute to cancer therapy.

Publication Date

2018

Subject Major(s)

Hematology, Oncology

Keywords

Acute Myeloid Leukemia, AML, Mefloquine, Drug Repurposing, Autophagy Inhibition, Apoptosis Induction

Current Academic Year

Senior

Faculty Advisor/Mentor

Dr. Seth Corey

Rights

© The Author(s)

Disciplines

Chemical Actions and Uses | Hematology | Hemic and Lymphatic Diseases | Medical Pathology | Oncology | Pediatrics

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