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Abstract
The aim of this study is to observe cell proliferation, cell viability, apoptosis, and autophagy on acute myeloid leukemia (AML) cell lines, NB4 and U937, with the drug repurposing of mefloquine (MQ). Methods such as the 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay and trypan blue staining have shown a decrease in live cells with high concentrations of mefloquine. Using their average perspective IC50 values of MQ concentration, Western blotting was applied by means of apoptosis and autophagy markers to determine if the induction of apoptosis and inhibition of autophagy was present in MQ-treated AML cells. The experiment will be continued with more cell lines, drugs, and other means of protocol in order to contribute to cancer therapy.
Publication Date
2018
Subject Major(s)
Hematology, Oncology
Keywords
Acute Myeloid Leukemia, AML, Mefloquine, Drug Repurposing, Autophagy Inhibition, Apoptosis Induction
Disciplines
Chemical Actions and Uses | Hematology | Hemic and Lymphatic Diseases | Medical Pathology | Oncology | Pediatrics
Current Academic Year
Senior
Faculty Advisor/Mentor
Dr. Seth Corey
Rights
© The Author(s)
Recommended Citation
[1] Arakawa, S., S. Honda, H. Yamaguchi, and S. Shimizu. 2017 Molecular mechanisms and physiological roles of Atg5/Atg7-independent alternative autophagy. Journal List. 93(6): 378–385. [2] Eriksson, A., E. Chantzi, M. Fryknäs, J. Gullbo, P. Nygren, et al. 2017 Towards repositioning of quinacrine for treatment of acute myeloid leukemia - Promising synergies and in vivo effects. Leukemia research. 63:41-46. [3] Folkerts, H., S. Hilgendorf, A.T.J. Wierenga, J. Jaques, A. B. Mulder et al. 2017 Inhibition of autophagy as a treatment strategy for p53 wild-type acute myeloid leukemia. Cell Death and Disease. 8(7):e2927. [4] Sharma, N., S. Thomas, E.B. Golden, F. M. Hofman, T. C. Chen et al. 2012 Inhibition of autophagy and induction of breast cancer cell death by mefloquine, an antimalarial agent. Cancer Letters. 326(2):143-54 [5] Staines, H.M., B.C. Dee, M.R. Shen, and J.C. Ellory. 2004 The effect of mefloquine and volume-regulated anion channel inhibitors on induced transport in Plasmodium falciparum-infected human red blood cells. Blood Cells Mol Dis. 32(3):344-8
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Chemical Actions and Uses Commons, Hematology Commons, Hemic and Lymphatic Diseases Commons, Medical Pathology Commons, Oncology Commons, Pediatrics Commons