Document Type

STEM

Date

2023

Submission Date

November 2023

Abstract

Composed of sphingosine and a fatty acid, ceramides are lipid molecules that serve as key metabolic signaling molecules of a sphingolipid pathway. While it acts as a precursor of complex sphingolipids, inducing ceramide generation can cause cell stress leading to subsequent cell death via apoptosis, necrosis, and even mitophagy. With regards to cell death specifically, a novel form of regulated cell death, ferroptosis, has recently been recognized of necrotic nature. Its unique morphological features and distinct properties have been observed over the last several decades; however, the molecular features were not identifiable as pure evidence of cell death, until recently as of 2012. This process is entirely iron-dependent with the accumulation of lipid peroxides that result in cell death. Small molecules with RAS-selective lethality have been found to be a ferroptosis-triggering agent due to the increase in reactive oxygen species (ROS) and iron levels. The present study attempts to highlight the role of the sphingolipid pathway in ferroptosis. Specifically, the study will evaluate the role of Ceramide Synthase (CerS) in ferroptosis by measuring changes in CerS6 levels and activity following RSL3-induced ferroptosis.

Rights

© The Author(s)

Is Part Of

Auctus

DOI

https://doi.org/10.25886/0sjw-1450

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