Document Type
Article
Original Publication Date
2015
Journal/Book/Conference Title
PLoS ONE
Volume
10
Issue
5
DOI of Original Publication
10.1371/journal.pone.0092091
Date of Submission
November 2015
Abstract
The complete sequence characterization of snake venom proteins by mass spectrometry is rather challenging due to the presence of multiple isoforms from different protein families. In the present study, we investigated the tryptic digest of the venom of the viperid snake Sistrurus catenatus edwardsii by a combined approach of liquid chromatography coupled to either electrospray (online) or MALDI (offline) mass spectrometry. These different ionization techniques proved to be complementary allowing the identification a great variety of isoforms of diverse snake venom protein families, as evidenced by the detection of the corresponding unique peptides. For example, ten out of eleven predicted isoforms of serine proteinases of the venom of S. c. edwardsii were distinguished using this approach. Moreover, snake venom protein families not encountered in a previous transcriptome study of the venom gland of this snake were identified. In essence, our results support the notion that complementary ionization techniques of mass spectrometry allow for the detection of even subtle sequence differences of snake venom proteins, which is fundamental for future structure-function relationship and possible drug design studies.
Rights
Copyright: © 2015 Chapeaurouge et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Is Part Of
VCU Biochemistry and Molecular Biology Publications
Sequences of venom proteins from S. c. edwardsii identified by tandem mass spectrometry.
journal.pone.0092091.s002.DOC (100 kB)
Comparison of the sequences determined by de novo sequencing of MS/MS spectra of the venom of S. edwardsii (Sanz et al.) with the sequences detected in the present study.
journal.pone.0092091.s003.DOC (38 kB)
Comparison of the sequences determined by Edmann sequencing of the venom of S. edwardsii (Sanz et al.) with the sequences detected in the present study.
journal.pone.0092091.s004.DOCX (87 kB)
Sequence coverages of some of the predicted venom gland proteins of S. c. edwardsii as revealed by the combined approach of MALDI and ESI tandem mass spectrometry.
journal.pone.0092091.s005.DOCX (53 kB)
Sequence coverages of some of the predicted venom gland proteins of S. c. edwardsii as revealed by the combined approach of MALDI and ESI tandem mass spectrometry.
Comments
Originally published at http://dx.doi.org/10.1371/journal.pone.0092091