Document Type

Article

Original Publication Date

2016

Journal/Book/Conference Title

PLOS ONE

Volume

11

Issue

7

DOI of Original Publication

10.1371/journal.pone.0159601

Comments

Originally published at http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0159601.

Funded in part by the VCU Libraries Open Access Publishing Fund.

Date of Submission

November 2016

Abstract

Identification of dysregulated microRNAs (miRNAs) in prostate cancer is critical not only for diagnosis, but also differentiation between the aggressive and indolent forms of the disease. miR-9 was identified as an oncomiR through both miRNA panel RT-qPCR as well as high-throughput sequencing analysis of the human P69 prostate cell line as compared to its highly tumorigenic and metastatic subline M12, and found to be consistently upregulated in other prostate cell lines including DU-145 and PC3. While miR-9 has been characterized as dysregulated either as an oncomiR or tumour suppressor in a variety of other cancers including breast, ovarian, and nasopharyngeal carcinomas, it has not been previously evaluated and proven as an oncomiR in prostate cancer. miR-9 was confirmed an oncomiR when found to be overexpressed in tumour tissue as compared to adjacent benign glandular epithelium through laser-capture microdissection of radical prostatectomy biopsies. Inhibition of miR-9 resulted in reduced migratory and invasive potential of the M12 cell line, and reduced tumour growth and metastases in male athymic nude mice. Analysis showed that miR-9 targets e-cadherin and suppressor of cytokine signalling 5 (SOCS5), but not NF-ĸB mRNA. Expression of these proteins was shown to be affected by modulation in expression of miR-9.

Rights

Copyright: © 2016 Seashols-Williams et al.

Is Part Of

VCU Biochemistry and Molecular Biology Publications

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