Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF

Authors

Harold Brem, New York University School of Medicine
Michael S. Golinko, New York University School of Medicine
Olivera Stojadinovic, Cornell University
Arber Kodra, New York University School of Medicine
Robert F. Diegelmann, Virginia Commonwealth UniversityFollow
Sasa Vukelic, Cornell University
Hyacinth Entero, Ross University School of Medicine
Donald L. Coppock, Coriell Institute for Medical Research
Marjana Tomic-Canic, Cornell University

Document Type

Article

Original Publication Date

2008

Journal/Book/Conference Title

Journal of Translational Medicine

Volume

6

DOI of Original Publication

10.1186/1479-5876-6-75

Comments

Originally published at http://dx.doi.org/10.1186/1479-5876-6-75

Date of Submission

September 2014

Abstract

Background Multiple physiologic impairments are responsible for chronic wounds. A cell line grown which retains its phenotype from patient wounds would provide means of testing new therapies. Clinical information on patients from whom cells were grown can provide insights into mechanisms of specific disease such as diabetes or biological processes such as aging.

The objective of this study was 1) To culture human cells derived from patients with chronic wounds and to test the effects of putative therapies, Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) on these cells. 2) To describe a methodology to create fibroblast cell lines from patients with chronic wounds.

Methods Patient biopsies were obtained from 3 distinct locations on venous ulcers. Fibroblasts derived from different wound locations were tested for their migration capacities without stimulators and in response to GM-CSF. Another portion of the patient biopsy was used to develop primary fibroblast cultures after rigorous passage and antimicrobial testing.

Results Fibroblasts from the non-healing edge had almost no migration capacity, wound base fibroblasts were intermediate, and fibroblasts derived from the healing edge had a capacity to migrate similar to healthy, normal, primary dermal fibroblasts. Non-healing edge fibroblasts did not respond to GM-CSF. Six fibroblast cell lines are currently available at the National Institute on Aging (NIA) Cell Repository.

Conclusion We conclude that primary cells from chronic ulcers can be established in culture and that they maintain their in vivo phenotype. These cells can be utilized for evaluating the effects of wound healing stimulators in vitro.

Rights

© 2008 Brem et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Is Part Of

VCU Biochemistry and Molecular Biology Publications