The interactome of Streptococcus pneumoniae and its bacteriophages show highly specific patterns of interactions among bacteria and their phages

Authors

Rachelle Mariano, University of Miami
Stefan Wuchty, University of Miami
Maria G. Vizoso-Pinto, Ludwig Maximilians-University, Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICETUNT, and Instituto de Fisiología
Roman Häuser, German Cancer Research Center
Peter Uetz, Virginia Commonwealth UniversityFollow

Document Type

Article

Original Publication Date

2016

Journal/Book/Conference Title

Scientific Reports

Volume

6

Issue

2016

DOI of Original Publication

10.1038/srep24597

Comments

Originally published at http://dx.doi.org/10.1038/srep24597.

Funded in part by the VCU Libraries Open Access Publishing Fund.

Date of Submission

February 2017

Abstract

Although an abundance of bacteriophages exists, little is known about interactions between their proteins and those of their bacterial hosts. Here, we experimentally determined the phage-host interactomes of the phages Dp-1 and Cp-1 and their underlying protein interaction network in the host Streptococcus pneumoniae. We compared our results to the interaction patterns of E. coli phages lambda and T7. Dp-1 and Cp-1 target highly connected host proteins, occupy central network positions, and reach many protein clusters through the interactions of their targets. In turn, lambda and T7 targets cluster to conserved and essential proteins in E. coli, while such patterns were largely absent in S. pneumoniae. Furthermore, targets in E. coli were mutually strongly intertwined, while targets of Dp-1 and Cp-1 were strongly connected through essential and orthologous proteins in their immediate network vicinity. In both phage-host systems, the impact of phages on their protein targets appears to extend from their network neighbors, since proteins that interact with phage targets were located in central network positions, have a strong topologically disruptive effect and touch complexes with high functional heterogeneity. Such observations suggest that the phages, biological impact is accomplished through a surprisingly limited topological reach of their targets.

Rights

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Is Part Of

VCU Study of Biological Complexity Publications