DOI

https://doi.org/10.25772/97C5-2M97

Defense Date

2014

Document Type

Thesis

Degree Name

Master of Science

Department

Human Genetics

First Advisor

Joe Landry

Abstract

Genomic imprinting results in monoallelic parent-of-origin gene expression. Therian mammals show conclusive evidence for imprinting, while the evidence in Aves is conflicting. It’s unclear if Aves have the proteins necessary for establishment and maintenance of imprinting loci. Every examined avian orthologue to mammalian imprinted genes shows biallelic expression providing evidence for a lack of imprinting in Aves. While the known parent-of-origin quantitative trait loci in chicken do not overlap with differentiated methylated regions, further analysis with a larger sample size is required. No transcript in the chicken transcriptome at incubation day 4.5 shows parent-of-origin expression, providing strong evidence for a lack of imprinting at this stage of development. Investigating expression of the chicken transcriptome at additional developmental time points, and the transcriptome of other Aves would provide decisive evidence on the presence or lack of imprinting in Aves. Based on current knowledge, Aves lack imprinting as observed in mammals.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

May 2014

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