DOI
https://doi.org/10.25772/CAM4-XC27
Defense Date
2016
Document Type
Thesis
Degree Name
Master of Science
Department
Human Genetics
First Advisor
Joseph Landry
Abstract
The nucleosome remodeling factor (NURF) is a chromatin remodeling complex involved in early animal development and is implicated in a number of cancers. In previous work, knockdown of NURF’s largest subunit, BPTF, resulted in diminished tumor growth in mouse cancer cell lines. Other studies in our lab demonstrated increased activation of T-lymphocytes into BPTF KD tumors. In order to examine if this approach has any therapeutic potential, this work investigates the effects of BPTF knockdown in established tumors by using recombinant adenoviruses (rAd), as well as observe the way the immune system interacts with BPTF knockdown cells, both in vivo by flow cytometry and in culture with cytotoxicity assays.
Rights
© The Author
Is Part Of
VCU University Archives
Is Part Of
VCU Theses and Dissertations
Date of Submission
5-16-2016