DOI
https://doi.org/10.25772/QG99-3D07
Defense Date
2018
Document Type
Thesis
Degree Name
Master of Science in Dentistry
Department
Dentistry
First Advisor
Dr. Zhao Lin
Second Advisor
Dr. Thomas Waldrop
Third Advisor
Dr. Harvey Schenkein
Abstract
Background: Exosomes have great potential in regenerative medicine through the
transfer of their bioactive cargos, such as RNA. tRF RNA and tiRNA are tRNAderived
non-coding RNA. Here, we sought to identify the tRF/tiRNA profile in human
mesenchymal stem cell (hMSC) exosomes. Methods: Bone marrow hMSCs were
cultured with/without osteogenic differentiation medium and exosomes were
harvested. RNA was extracted from: 1) control cells (Cell-NT); 2) control exosomes
(EXO-NT); 3) differentiated cells (Cell-OM); 4) exosomes produced by differentiated
cells (EXO-OM). RNA was sequenced to profile the small RNA with a focus on
tRF/tiRNA. Results: tRF/tiRNA was highly enriched in hMSC exosomes. Less
diversity was seen in the tRF/tiRNA profile in exosomes than that in parent cells.
Selective tRF/tiRNA were packed into MSC exosomes and their profile is dependent
on the cell maturation status. Conclusions: Our results suggest that tRF/tiRNA may
play a role in mediating the function of exosomes in tissue regeneration.
Rights
© The Author
Is Part Of
VCU University Archives
Is Part Of
VCU Theses and Dissertations
Date of Submission
11-12-2018