DOI
https://doi.org/10.25772/QVFE-VD26
Author ORCID Identifier
https://orcid.org/0000-0002-4598-9399
Defense Date
2017
Document Type
Dissertation
Degree Name
Doctor of Philosophy
Department
Biomedical Engineering
First Advisor
Zvi Schwartz, D.M.D., Ph.D.
Second Advisor
Barbara D. Boyan, Ph.D.
Third Advisor
Christopher Lemmon, Ph.D.
Fourth Advisor
Christina Tang, Ph.D.
Fifth Advisor
Vamsi Yadavalli, Ph.D.
Sixth Advisor
Hu Yang, Ph.D.
Abstract
The goal of this research was to develop a system of individualized medicine that could be applied to dermal wounds serving as a wound dressing and synthetic extracellular matrix while delivering stem cells to the wound bed. First, fabrication parameters for electrospinning polymer fibers were determined. This involved evaluating fiber morphology with respect to polymer selection and solution concentration. Next, construct fabrication was examined to produce an integrated void space, or cargo area, suitable to maintain stem cells. In vitro studies to ensure stem cell viability and phenotype were conducted, and results supported the notion that cells could be administered to the wound site through construct pre-seeding. Lastly, in vivostudies were conducted to evaluate the construct as an applied biomaterial and as a cellular delivery device. Wound closure and quality were assessed, and neo-vascularization quantified. This project will provide insight into the tissue engineering field regarding cell-based therapies and dermal wound healing.
Rights
© Ryan Clohessy
Is Part Of
VCU University Archives
Is Part Of
VCU Theses and Dissertations
Date of Submission
8-9-2019
Included in
Biomaterials Commons, Biomedical Devices and Instrumentation Commons, Molecular, Cellular, and Tissue Engineering Commons