DOI
https://doi.org/10.25772/QJ8W-QM39
Defense Date
2021
Document Type
Thesis
Degree Name
Master of Science
Department
Physiology and Biophysics
First Advisor
Dr. Stefano Toldo
Second Advisor
Dr. Antonio Abbate
Third Advisor
Dr. Roland Pittman
Abstract
Heart failure (HF) is characterized by dyspnea, fatigue, and exercise intolerance. Clinical evidence points to increased interleukin-1β (IL-1β) activity in patients with HF, with an IL-1 blockade improving the exercise capacity in HF patients. In healthy mice, recombinant-mouse IL-1β (rmIL-1β) induces acute systolic dysfunction, peaking 4 hours after administration. However, the direct effects of rmIL-1β on exercise capacity are unknown. We hypothesized that rmIL-1β diminishes the exercise capacity in the mouse. Adult mice were trained to run on a treadmill and exercise capacity was assessed before, 4 hours, and 96 hours after intraperitoneal administration of rmIL-1β (3 μg/kg) or vehicle (0.9% NaCl) (N=7-10/group). In separate mice, left ventricular ejection fraction (LVEF) was assessed, at the same time points, after administration of rmIL-1β or vehicle using transthoracic echocardiography. The ultrasound operator was blinded to treatments. The cardiac reserve was measured by calculating the difference in LVEF before and after β-adrenergic stimulation using isoproterenol (10 ng/mouse) (N=5-11/group). Treatment with rmIL-1β significantly reduced exercise capacity after 4 hours and 96 hours (P
Rights
© Habeebah Z. Vohra
Is Part Of
VCU University Archives
Is Part Of
VCU Theses and Dissertations
Date of Submission
8-11-2021