DOI

https://doi.org/10.25772/H11E-ZZ40

Author ORCID Identifier

0000-0002-3140-9605

Defense Date

2025

Document Type

Thesis

Degree Name

Master of Science

Department

Psychology

First Advisor

Caroline Cobb

Second Advisor

Eric Benotsch

Third Advisor

M. Imad Damaj

Abstract

Research on tobacco product standards to limit the abuse liability of electronic nicotine delivery systems (ENDS) has primarily focused on nicotine concentration and flavor. Other liquid characteristics, such as protonated nicotine ratio and sweetener additives, are less understood but may serve as regulatory targets. This clinical laboratory study examined the effects of ENDS protonated nicotine ratio and sweetener presence on nicotine delivery, use behavior, and subjective effects.

Thirteen participants completed four sessions that varied by protonated nicotine ratio (0:100 vs. 40:60 freebase to monoprotonated nicotine) and sweetener status (sweetened with 3 mg/mL ethyl maltol vs. unsweetened). Each session included a 10-puff directed use period and a 30-minute ad libitum use period, followed by an own-brand challenge. Measures included heart rate, subjective effects, plasma nicotine concentration, and liquid consumption.

Results showed that nicotine form significantly influenced nicotine delivery, use behavior, and subjective effects. The 0:100 protonation ratio produced greater nicotine boost, longer puff duration, and larger puff volume compared to the 40:60 condition. Sweetener did not impact nicotine delivery but enhanced flavor perception and appeal when combined with the 0:100 ratio. Unsweetened liquids were associated with greater nausea, while 40:60 conditions were perceived as harsher and more irritating. The 0:100 liquids increased concentration ratings, whereas 40:60 liquids elicited greater immediate desire to use again.

These findings underscore the need for ENDS product standards to consider not only nicotine concentration but also nicotine form and sweetener presence to more effectively reduce abuse liability.

Rights

© The Author

Is Part Of

VCU University Archives

Is Part Of

VCU Theses and Dissertations

Date of Submission

4-28-2025

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