Document Type
Article
Original Publication Date
2015
Journal/Book/Conference Title
Magnetic Resonance in Medicine
Volume
74
Issue
2
DOI of Original Publication
10.1002/mrm.25748
Date of Submission
December 2015
Abstract
Purpose
To develop an imaging tool that enables the detection of malignant tissue with enhanced specificity using the exquisite spatial resolution of MRI.
Methods
Two mammalian gene expression vectors were created for the expression of the lysine-rich protein (LRP) under the control of the cytomegalovirus (CMV) promoter and the progression elevated gene-3 promoter (PEG-3 promoter) for constitutive and tumor-specific expression of LRP, respectively. Using those vectors, stable cell lines of rat 9L glioma, 9LCMV-LRP and 9LPEG-LRP, were established and tested for CEST contrast in vitro and in vivo.
Results
9LPEG-LRP cells showed increased CEST contrast compared with 9L cells in vitro. Both 9LCMV-LRP and 9LPEG-LRP cells were capable of generating tumors in the brains of mice, with a similar growth rate to tumors derived from wild-type 9L cells. An increase in CEST contrast was clearly visible in tumors derived from both 9LCMV-LRP and 9LPEG-LRP cells at 3.4 ppm.
Conclusion
The PEG-3 promoter:LRP system can be used as a cancer-specific, molecular-genetic imaging reporter system in vivo. Because of the ubiquity of MR imaging in clinical practice, sensors of this class can be used to translate molecular-genetic imaging rapidly.
Rights
Copyright © 2015 Wiley Periodicals, Inc.
Is Part Of
VCU Human and Molecular Genetics Publications
Comments
Originally published at http://dx.doi.org/10.1002/mrm.25748