Document Type
Article
Original Publication Date
2018
Journal/Book/Conference Title
Cell Reports
Volume
22
Issue
7
First Page
1824
Last Page
1834
DOI
10.1016/j.celrep.2018.01.048
Date of Submission
October 2019
Abstract
Helminth infection is known for generating large amounts of poly-specific IgE. Here we demonstrate that innate-like B1 cells are responsible for this IgE production during infection with the nematode parasites Nippostrongylus brasiliensis and Heligmosomoides polygyrus bakeri. In vitro analysis of B1 cell immunoglobulin class switch recombination to IgE demonstrated a requirement for anti-CD40 and IL-4 that was further enhanced when IL-5 was added or when the B1 source was helminth infected mice. An IL-25-induced upregulation of IgE in B1 cells was also demonstrated. In T cell-reconstituted RAG1−/− mice, N. brasiliensis clearance was enhanced with the addition of B2 cells in an IgE-dependent manner. This enhanced clearance was impeded by reconstitution with IgE sufficient B1 cells. Mucosal mast cells mediated the B2 cell enhancement of clearance in the absence of B1 cells. The data support B1 cell IgE secretion as a regulatory response exploited by the helminth.
Rights
© 2018 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Is Part Of
VCU Microbiology and Immunology Publications
Comments
Originally published at https://doi.org/10.1016/j.celrep.2018.01.048
Funded in part by the VCU Libraries Open Access Publishing Fund.