Document Type
Article Presentation
Original Publication Date
2024
Date of Submission
May 2024
Abstract
Wnt7a serves an important role in slowing down the growth of non-small-cell lung cancer (NSCLC). However, little is known about the effect(s) of Wnt7a on the sensitivity of NSCLC to radiation. In the study, NSCLC cell proliferation and apoptosis in response to Wnt7a overexpression and/or radiation as one combination were determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-tertazolium bromide (MTT) assay and flow cytometry, respectively. The western blot technique was used to further examine the activation of Wnt/cJun N-terminal kinase (JNK) and Wnt/b-catenin signaling pathways in NSCLC cell lines H1650 and A549.
The results indicate that the overexpression of Wnt7a, in combination with radiation inhibits cell proliferation and induces apoptosis in NSCLC cell lines compared to either one alone, Wnt7a overexpression or radiotherapy. The phosphorylation of JNK, in addition, rather than b-catenin, aligns with the changes in Wnt7a overexpression and/or radiation. The Wnt/JNK signaling pathway, when inhibited by a JNK inhibitor, SP600125, however, contributes to the proliferation induction in NSCLC cells. Considered collectively, the results show that overexpression of Wnt7a has the potential to make NSCLC cells more sensitive to radiation therapy through the Wnt/JNK pathway.
Rights
© The Author(s)
