Quantification of Biomass and Cell Motion in Human Pluripotent Stem Cell Colonies

Authors

Thomas A. Zangle, University of California - Los Angeles
Jennifer Chun, University of California - Los Angeles
Jin Zhang, University of California - Los Angeles
Jason C. Reed, University of California - Los Angeles, Virginia Commonwealth UniversityFollow
Michael A. Teitell, University of California - Los AngelesFollow

Document Type

Article

Original Publication Date

2013

Journal/Book/Conference Title

The Biophysical Journal

Volume

105

Issue

3

First Page

593

Last Page

601

DOI of Original Publication

10.1016/j.bpj.2013.06.041

Comments

Originally published at http://dx.dpi.org/10.1016/j.bpj.2013.06.041

Under an Elsevier user license

Date of Submission

February 2015

Abstract

Abstract

Somatic cell reprogramming to pluripotency requires an immediate increase in cell proliferation and reduction in cell size. It is unknown whether proliferation and biomass controls are similarly coordinated with early events during the differentiation of pluripotent stem cells (PSCs). This impasse exists because PSCs grow in tight clusters or colonies, precluding most quantifying approaches. Here, we investigate live cell interferometry as an approach to quantify the biomass and growth of HSF1 human PSC colonies before and during retinoic acid-induced differentiation. We also provide an approach for measuring the rate and coordination of intracolony mass redistribution in HSF1 clusters using live cell interferometry images. We show that HSF1 cells grow at a consistent, exponential rate regardless of colony size and display coordinated intracolony movement that ceases with the onset of differentiation. By contrast, growth and proliferation rates show a decrease of only ∼15% decrease during early differentiation despite global changes in gene expression and previously reported changes in energy metabolism. Overall, these results suggest that cell biomass and proliferation are regulated independent of pluripotency during early differentiation, which is distinct from what occurs with successful reprogramming.

Rights

From The Biophysical Journal, Zangle, T.A., Chun, J., Zhang, J., et al., Quantification of Biomass and Cell Motion in Human Pluripotent Stem Cell Colonies, Vol. 105, Page 593, Copyright © 2013 Biophysical Society. Published by Elsevier Inc. Reprinted with permission.

Is Part Of

VCU Physics Publications