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Abstract
ABSTRACT Virus induced gene silencing (VIGS) is an efficient technique to quickly evaluate gene functions. VIGS utilizes the mechanism(s) of RNA interference (RNAi), which the virus vector carrying the gene of interest will be silenced through posttranscriptional gene silencing. Agrobacterium is used to carry the constructed vectors to infect the plants . Here, I summarized several inoculation methods have been reported recently to infect the plants. We are working on evaluating the efficiency with these different methods in our study system. Our lab uses Nicotiana obtusifolia (desert tobacco) as a model to studygene regulating plant development. I report the result of leaf inoculation by using syringe. PDS gene was silenced show a phenotype with bleached leaves . We are working on evaluating the most effective inoculation methods for our system. VIGS, PDS, plasmid, agrobacterium, inoculation, vector, transmission
Publication Date
2014
Subject Major(s)
Genetics
Current Academic Year
Senior
Faculty Advisor/Mentor
Wenheng Zhang
Sponsorship
Virginia Commonwealth University. Undergraduate Research Opportunities Program
Is Part Of
VCU Undergraduate Research Posters
Rights
© The Author(s)