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Abstract
Volumetric muscle loss (VML) has been found to overwhelm muscle regeneration, resulting in loss of long-term muscle functionality. Decellularized muscle matrices (DMMs) provide an effective environment for muscle regeneration; however, the age of their source has not been adequately explored for clinical translation. Advanced glycation end-products (AGEs) are chemical cross-links that contribute to the aging process by accumulating on collagen fibers, resulting in a stiffening of the collagenous matrix and an increase in inflammation via the receptor for advanced glycation end-products (RAGE). In previous experiments, we found increased levels of AGE-specific cross-links within DMMs in old mice compared to young as proven by ALT-711 treatment. In this study, we developed a model of aged rat DMMs using AGE cross-links and hypothesized that our AGE-DMM model will contain a higher number of collagen cross-links compared to the control. This AGE-DMM model aims to elucidate the effect of AGEs on muscle regeneration when used in vitro or implanted in a volumetric muscle loss model.
Publication Date
2022
Keywords
decellularization; skeletal muscle aging; advanced glycation end-products; collagen; cross-linking
Current Academic Year
Senior
Faculty Advisor/Mentor
David Joshua Cohen
Faculty Advisor/Mentor
Barbara D. Boyan
Faculty Advisor/Mentor
Michael J. McClure
Faculty Advisor/Mentor
Zvi Schwartz
Rights
© The Author(s)