Document Type
Article
Original Publication Date
2012
Journal/Book/Conference Title
Evidence-Based Complementary and Alternative Medicine
Volume
2012
DOI of Original Publication
10.1155/2012/872458
Date of Submission
August 2014
Abstract
Many active components of herbal products are small organic anions, and organic anion transporters were previously demonstrated to be a potential site of drug-drug interactions. In this study, we assessed the inhibitory effects of six hydrophilic components of the herbal medicine Danshen, lithospermic acid, protocatechuic acid, rosmarinic acid, salvianolic acid A, salvianolic acid B, and tanshinol, on the function of the murine organic anion transporters, mOat1 and mOat3. All of Danshen components significantly inhibited mOat1- and mOat3-mediated substrate uptake () with lithospermic acid (LSA), protocatechuic acid, rosmarinic acid (RMA), and salvianolic acid A (SAA) producing virtually complete inhibition under test conditions. Kinetic analysis demonstrated that LSA, RMA, and SAA were competitive inhibitors. As such, values were estimated as μM for LSA, μM for RMA, and μM for SAA on mOat1-mediated transport, and as μM for LSA, μM for RMA, and μM for SAA on mOat3-mediated transport. These data suggest that herb-drug interactions may occur in vivo on the human orthologs of these transporters in situations of polypharmacy involving Danshen and clinical therapeutics known to be organic anion transporter substrates.
Rights
Copyright © 2012 Li Wang and Douglas H. Sweet. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Is Part Of
VCU Pharmaceutics Publications
Comments
Originally published at
http://dx.doi.org/10.1155/2012/872458